We took Luna to the vet for an ultrasound yesterday hoping to confirm a pregnancy. She was bred to Gil on March 14th and we were hoping to have a litter on the ground towards the end of May. Unfortunately it was not to be; the vet was unable to find any puppies, and we were understandably disappointed. The vet suggested that we do a complete thyroid panel and cultures on Luna to make sure there's nothing wrong on that end. Given her age, and the fact that she's a virgin bitch, we may have to use a more invasive breeding method next time around.